Glutinol inhibits the proliferation of human ovarian cancer cells via PI3K/AKT signaling pathway


 Purpose: To investigate the anticancer effect of glutinol on OVACAR3 human ovarian cancer cells, and to elucidate underlying molecular mechanisms.
 Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. cycle distribution, reactive oxygen species (ROS) mitochondrial membrane potential were measured via flow cytometry, while protein expression levels with western blotting assay.
 Results: Glutinol exerted significant growth-inhibitory effects but interestingly, it exhibited comparatively lower cytotoxic against normal SV40 cells. The IC50 cells 6 ?M, 60 ?M. Flow cytometric analysis showed an increase in population G2/M phase from 4.02 % control 29.05 treatment 12 ?M glutinol, suggestive arrest. arrest also accompanied by suppression cyclin B1. It found that increases ROS decreases MMP activities contributed glutinol-induced antiproliferative Moreover, deactivated PI3K/AKT signaling pathway OVACAR-3 cells.
 Conclusion: potent cancer. Thus, might be benefit cancer.